Cells were dissociated with Accutase with 10 units/mL of papain (Sigma-Aldrich) for 10 minutes at 37°C and resuspended in 0.5% bovine serum albumin (Sigma Aldrich) with 50 units/mL DNase I in DPBS ...
EBs were collected at d2, d4, and d6 time-points. To cellularise, 20 pooled EBs were incubated in 1ml accutase for 12min in a 15ml falcon tube with gentle shaking before being quenched with wash ...
Cells were then washed and collected with accutase (Thermo Fisher Scientific, Waltham, MA, USA) treatment and seeded at 50,000 cells/well in a 96-well U-bottom plate (Sarstedt AG & Co. KG, Nümbrecht, ...
Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and ...
Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and ...
Background: Pancreatic cancer (PC), sometimes referred to as pancreatic ductal adenocarcinoma (PDAC), is a major cause of global mortality from cancer. Pancreatic cancer is a very aggressive and ...